Florida Department of Education
Curriculum Framework 

The standards and benchmarks for this program were updated for the 2026-27 academic year.

Program Title: 	Medical Laboratory Technology
Career Cluster: 	Health Science

Associate in Science	
CIP Number	1351100405	
Program Type	College Credit	
Program Length	76 credit hours	
CTSO	HOSA	
SOC Codes (all applicable) 	For program SOC codes, please see the Program and Course Tables section of the CTE Program Resources page linked below.	
CTE Program Resources 	http://www.fldoe.org/academics/career-adult-edu/career-tech-edu/program-resources.stml 	

Purpose

This program offers a sequence of courses that provides coherent and rigorous content aligned with challenging academic standards and relevant technical knowledge and skills needed to prepare for further education and careers in the Health Science career cluster; provides technical skill proficiency, and includes competency-based applied learning that contributes to the academic knowledge, higher-order reasoning and problem-solving skills, work attitudes, general employability skills, technical skills, and occupation-specific skills, and knowledge of all aspects of Health Science career cluster. 

The purpose of this program is to prepare students for employment as medical/clinical laboratory technologists (medical clinical laboratory technologist) or medical laboratory technologists (associate degree) or to provide supplemental training for persons previously or currently employed in these occupations.

The content includes, but is not limited to, specific techniques and instruments, identification of factors directly affecting procedures and results, confirmation of results, monitoring quality control programs within pre-determined parameters, and correction of errors using pre-set standards.  A clinical component is a necessary element of this program.

Additional Information relevant to this Career and Technical Education (CTE) program is provided at the end of this document.

Program Structure

This program is a planned sequence of instruction consisting of 76 credit hours.

When developing the course sequence for the local implementation of this program, select courses that:
•	Meet the skill development requirements of the standards and benchmarks of this program.
•	Meet the general education core course requirements of Rule 6A-14.0303, Florida Administrative Code.
•	Where reasonable and practicable, align with programs offered by your institution or other postsecondary institutions in Florida, including: 
o	Related CCC and ATD credentials.
o	Baccalaureate degrees within the same career pathway and/or Federal CIP code.

Visit FloridaShines’ Discover Programs website to identify other institutions that offer similar programs to review their course sequencing. Where appropriate, course alignment between institutions offering related programs can facilitate interagency student articulation.

Visit FloridaShines’ Common Prerequisites Manual to identify courses that are prerequisites for admission into an upper division program.  When sub-baccalaureate courses are aligned to the course admissions requirements of baccalaureate programs with the same career pathway, students can enter upper division coursework seamlessly.   

Regulated Programs

This program is regulated by the Florida Board of Clinical Laboratory Personnel: http://floridasclinicallabs.gov/

Florida’s Career Readiness Skills for CTE Programs

Employability Skills	
01.0	Apply academic skills to workplace scenarios.	
01.01	Use reading skills.	
01.02	Use writing skills.	
01.03	Use mathematical strategies and procedures.	
01.04	Use scientific principles and procedures.	
02.0	Design a solution to an industry problem.	
02.01	Use critical thinking.	
02.02	Use creativity.	
02.03	Make sound decisions.	
02.04	Solve problems.	
02.05	Reason. 	
02.06	Plan and organize.	
03.0	Manage resources within an industry project	
03.01	Manage time.	
03.02	Manage money or resources.	
03.03	Manage materials.	
03.04	Manage personnel.	
04.0	Oversee the subcomponents, operations and output of a technical or organizational system.	
04.01	Manage systems.	
04.02	Monitor systems.	
04.03	Improve systems.	
05.0	Use information for decision making.	
05.01	Locate information.	
05.02	Organize information.	
05.03	Use information.	
05.04	Analyze information.	
05.05	Communicate information.	
06.0	Apply relevant technology to workplace scenarios to aid productivity.	
06.01	Use technology.	
07.0	Interpret and express interpersonal communication.	
07.01	Communicate verbally.	
07.02	Listen actively.	
07.03	Comprehend written material.	
07.04	Convey information in writing.	
07.05	Communicate nonverbally.	
07.06	Interpret nonverbal communication.	
08.0	Interact with others to accomplish workplace goals.	
08.01	Collaborate with others in a team.	
08.02	Respond to customer needs.	
08.03	Exercise leadership.	
08.04	Negotiate to resolve conflict.	
08.05	Respect others.	
09.0	Manage personal behavior to maximize productivity and professional growth.	
09.01	Demonstrate responsibility and self-discipline.	
09.02	Adapt and show flexibility.	
09.03	Work independently.	
09.04	Demonstrate a willingness to learn.	
09.05	Demonstrate integrity.	
09.06	Demonstrate professionalism.	
09.07	Take initiative.	
09.08	Display positive attitude.	
09.09	Take responsibility for professional growth.	
Job Attainment	
10.0	Find, assess and apply to job opportunities.	
10.01	Identify online job posts relevant to his or her career aspirations.	
10.02	Compare and contrast the job posts’ required qualifications, job duties, compensation, benefits and employers. 	
10.03	Define what information, documentation and writing prompts are required for the positions.	
11.0	Communicate personal competence, character and fit for a job opportunity.	
11.01	Develop a resume.	
11.02	Write a cover letter.	
11.03	Curate a professional portfolio that includes work products.	
11.04	Prepare for and experience a mock job interview.	
12.0	Cultivate and leverage relationships to professionally advance.	
12.01	Request a signed reference letter, letter of recommendation and/or an online skill/professionalism endorsement. 	
12.02	Develop a plan to cultivate a professional digital footprint.	
12.03	Develop a networking plan for a specific industry of interest.	

Standards

After successfully completing this program, the student will be able to perform the following:

01.0	Demonstrate knowledge of the healthcare delivery system and health occupations.
02.0	Demonstrate the ability to communicate and use interpersonal skills effectively.
03.0	Demonstrate legal and ethical responsibilities.
04.0	Demonstrate an understanding of and apply wellness and disease concepts.
05.0	Recognize and practice safety and security procedures.
06.0	Recognize and respond to emergency situations.
07.0	Recognize and practice infection control procedures.
08.0	Demonstrate an understanding of information technology applications in healthcare.
09.0	Demonstrate employability skills.
10.0	Demonstrate knowledge of bloodborne diseases, including HIV/AIDS.
11.0	Apply basic math and science skills. 
12.0	Demonstrate accepted professional, communication, and interpersonal skills.
13.0	Discuss phlebotomy in relation to the healthcare setting.
14.0	Identify the anatomic structure and function of body systems in relation to services performed by the phlebotomist.
15.0	Recognize and identify collection reagents supplies, equipment, and interfering chemical substances.
16.0	Demonstrate skills and knowledge necessary to perform phlebotomy.
17.0	Practice infection control following standard precautions.
18.0	Practice accepted procedures of transporting, accessioning, and processing specimens.
19.0	Practice quality assurance and safety.
20.0	Demonstrate knowledge and use of basic laboratory equipment and techniques.
21.0	Demonstrate basic knowledge of and perform clinical laboratory Point-of-Care (POC) testing (Waived).
22.0	Demonstrate basic knowledge of and perform Point-of-Care (POC) Testing using CLIA-approved waived instrumentation. 
23.0	Discuss the general responsibilities and functions encountered by a medical laboratory technician.
24.0	Apply quality assurance principles and protocols for laboratory equipment.
25.0	Demonstrate knowledge of the operation of computer systems.
26.0	Demonstrate an understanding of the basic principles of molecular diagnostics.
27.0	Demonstrate knowledge of urinalysis and body fluids principles and procedures.
28.0	Demonstrate knowledge of hematological principles and procedures.
29.0	Demonstrate knowledge of hemostasis and related diagnostic principles and procedures.
30.0	Demonstrate knowledge of immunology principles and procedures.
31.0	Demonstrate knowledge of clinical chemistry principles and procedures.
32.0	Demonstrate knowledge of immunohematology principles and procedures.
33.0	Demonstrate knowledge of microbiological principles and procedures.
34.0	Demonstrate knowledge of advanced hematological principles and procedures.
35.0	Demonstrate knowledge of advanced hemostasis testing. 
36.0	Demonstrate knowledge of advanced microbiological principles and procedures.
37.0	Demonstrate knowledge of advanced clinical chemistry principles and procedures.
38.0	Demonstrate knowledge of advanced immunological procedures.
39.0	Demonstrate knowledge of advanced immunohematology principles and procedures.
40.0	Demonstrate an understanding of advanced principles of molecular diagnostics.

Florida Department of Education
Student Performance Standards

Program Title: 	Medical Laboratory Technology 
CIP Number: 		1351100405  
Program Length: 	76 credit hours

Standards 1-11 are referred to as the Health Science Core and are required standards in this program. Secondary and Postsecondary students completing the health science core will not have to repeat the core in any other health science program in which it is a part.  When the recommended sequence is followed, the structure allows students to complete at specified points for employment or remain for advanced training or cross-training.  

To ensure consistency whenever these courses are offered, the health science core standards (1-11) and benchmarks have been placed in a separate document. To access this document, visit this link: 
https://www.fldoe.org/core/fileparse.php/20569/urlt/health-sci-core-psav-cc-2324.rtf

At the completion of this program, the student will be able to:	
12.0	Demonstrate accepted professional, communication, and interpersonal skills. The student will be able to:	
12.01	Demonstrate the appropriate professional behavior of a phlebotomist and describe the scope of practice.	
12.02	Explain to the patient the procedure to be used in specimen collection. 	
12.03	Explain in detail the importance of identifying patients correctly when drawing blood.	
12.04	Explain the importance of continuing education in relation to certification to maintain competency and skills.	
13.0	Discuss phlebotomy in relation to the healthcare setting. The student will be able to:	
13.01	List, classify, and discuss various departments and services within the health care setting in which the phlebotomist must interact with to obtain laboratory specimens from patients.	
13.02	Identify the major departments/sections with the clinical laboratory, the major types of procedures run in each department/section, and their specimen requirements.	
13.03	Describe roles of the major classifications of clinical laboratory personnel (i.e., pathologist, chief/administrative technologist, CLS, MLS, MLT, MT, phlebotomist, lab assistant, etc.).	
14.0	Identify the anatomic structure and function of body systems in relation to services performed by the phlebotomist. The student will be able to:	
14.01	Describe and define major body systems with emphasis on the circulatory system.	
14.02	List and describe the main superficial veins used in performing venipuncture.	
14.03	Locate the most appropriate sites(s) for capillary and venipuncture.	
14.04	Describe the function of the following blood components: erythrocytes, thrombocytes, leukocytes, and plasma.	
14.05	Compare and contrast serum and plasma as they relate to blood collection.	
14.06	Discuss hemostasis, hemolysis, and methods of prevention as they relate to blood collection.	
15.0	Recognize and identify collection reagents supplies, equipment, and interfering chemical substances. The student will be able to:	
15.01	Identify and discuss the proper use of appropriate types of equipment needed to collect various clinical laboratory blood specimens by venipuncture.	
15.02	Explain the special precautions and types of equipment needed to collect blood from the pediatric patient. 	
15.03	Identify and discuss the proper use of supplies used in collecting micro-specimens.	
15.04	Identify and discuss the proper use of the various types of anticoagulants, preservatives, and gels used in blood collection and the vacuum tube color-codes for these additives.	
15.05	Describe the types of specimens that are analyzed in the clinical laboratory and the phlebotomist's role in collecting and/or transporting these specimens to the laboratory.	
15.06	Describe substances potentially encountered during phlebotomy that can interfere with the analysis of blood constituents.	
15.07	Define and utilize correct medical terminology and metric measurement needed for specimen collection.	
16.0	Demonstrate skills and knowledge necessary to perform phlebotomy. The student will be able to:	
16.01	Follow approved procedure for completing a laboratory requisition form and recognize a properly completed form.	
16.02	Apply established protocol for patient and specimen identification for transport to a reference lab.	
16.03	Demonstrate knowledge of established protocol for patient and specimen identification.	
16.04	Discuss appropriate methods for facilitating and preparing the patient for capillary and venipuncture collection.	
16.05	List appropriate antiseptic agents useful in preparing sites for capillary and venipuncture for adult and pediatric patients.	
16.06	Know how to perform venipuncture by evacuated tube, butterfly, and syringe systems.	
16.07	Describe the correct order of draw. 	
16.08	Describe the use of barcoding systems used for positive patient identification and specimen identification.  	
16.09	Convey an understanding of capillary puncture using appropriate supplies and techniques for both adult and pediatric patients.	
16.10	Describe the most common complications associated with capillary and venipuncture, their causes, prevention, and treatment.	
16.11	Recognize and respond to possible adverse patient reactions such as allergies, convulsions, syncope, and lightheadedness.	
16.12	Perform appropriate procedures for disposing of used or contaminated capillary and venipuncture supplies.	
16.13	Perform appropriate techniques for making a peripheral blood smear for hematologic evaluation.	
16.14	Demonstrate the proper procedure for collecting blood cultures.	
16.15	Demonstrate a working understanding of how the age and weight of patients impact the maximum amount of blood that can be safely drawn.	
17.0	Practice infection control following standard precautions. The student will be able to:	
17.01	Demonstrate proper hand hygiene. 	
17.02	Comply with standard infection control precautions. 	
17.03	Identify potential routes of infection and their complications.	
18.0	Practice accepted procedures of transporting, accessioning, and processing specimens. The student will be able to:	
18.01	Follow the approved procedure for preparation and processing (e.g., centrifugation, separation, aliquoting, labeling, and storage) of serum, plasma, urine, sputum, stool, and wound culture specimens.  	
18.02	Demonstrate knowledge of accessioning procedures.	
18.03	Describe the importance of time considerations for specimen collection, transporting, and delivery.	
18.04	Describe routine procedures for transporting and processing specimens, including U.S. Department of Transportation packaging requirements.	
19.0	Practice quality assurance and safety. The student will be able to:	
19.01	Distinguish and perform procedures that ensure the reliability of test results when collecting laboratory specimens.	
19.02	Demonstrate knowledge of and practice appropriate patient safety.	
19.03	Practice safety in accordance with OSHA (State & Federal guidelines) for chemical, biological, and PPE established procedures, including proper disposal of sharps and biohazardous materials. 	
19.04	Follow policies and procedures for work-related accidents.	
19.05	Implement appropriate Joint Commission patient safety goals and other accrediting/regulatory agency guidelines.	
20.0	Demonstrate knowledge and use of basic laboratory equipment and techniques. The student will be able to:	
20.01	Identify the parts of the microscope and explain the function of each.	
20.02	Demonstrate the proper technique for use of the microscope.	
20.03	Demonstrate use of standard laboratory equipment, including pipetting skills and preventative maintenance.	
20.04	Perform basic laboratory math calculations, including serial dilutions and making solutions. 	
20.05	Understand the principles of quality assurance to correct problems encountered in monitoring daily quality control.	
20.06	Evaluate laboratory findings to confirm results according to standard operating procedure and best laboratory practices.	
20.07	Demonstrate knowledge of principles and operation of basic laboratory instruments.	
21.0	Demonstrate basic knowledge of and perform clinical laboratory Point-of-Care (POC) testing (Waived). The student will be able to:	
21.01  Demonstrate the ability to interpret instructions of point-of-care testing, including, but not limited to the following: Test principle
•	Storage and Stability
•	Internal vs. External Quality Control 
•	Specimen collection and preparation 
•	Directions for use 
•	Interpretation of results 
•	Interfering substances	
21.02	Demonstrate and discuss knowledge of lot numbers use and importance regarding both kits and reagents.	
21.03	 Demonstrate knowledge of the frequency of performing quality control procedures.	
21.04 Explain the Clinical Laboratory Improvement Amendments (CLIA) 88 classification of laboratory testing into waived, moderate, and highly complex, including the personnel qualified to perform each. 	
22.0	Demonstrate basic knowledge of and perform Point-of-Care (POC) testing using CLIA-approved waived instrumentation.  The student will be able to:	
22.01	Demonstrate and perform POC testing specific to microbiology, hematology, urinalysis, and clinical chemistry.	
22.02	Perform instrument maintenance.	
22.03	Demonstrate knowledge of quality control and calibrations involved with the POC instruments.	
22.04	Identify normal limits and associate abnormal results with disease or disorders.	
22.05	Discuss the significance of reporting critical values as it applies to (POC) testing as soon as possible.	
23.0	Discuss the general responsibilities and functions encountered by a medical laboratory technician. The students will be able to: 	
23.01	Ask appropriate scientific questions and recognize what is involved in experimental approaches to the solutions of such questions.	
23.02	Communicate laboratory results to healthcare professionals. 	
23.03	Demonstrate ability to evaluate laboratory results under the supervision of a medical laboratory scientist.  	
23.04	Demonstrate ability to report laboratory results in written or oral form under the supervision of a medical laboratory scientist.	
23.05	List licensure and certification requirements of the major classifications of clinical laboratory personnel.	
24.0	Apply quality assurance principles and protocols for laboratory equipment. The student will be able to: 	
24.01	Assess specimen acceptability using standard operating procedure, including rejection/recollection criteria. 	
24.02	Describe procedures for transporting and processing specimens.	
24.03	Describe the clinical laboratory role in providing quality assurance in laboratory testing, reporting, and the use and maintenance of equipment.	
24.04	Understand the need for calibration of laboratory equipment. 	
24.05	Demonstrate and record quality control procedures required for the tests performed and recognize unacceptable results.	
24.06	Report identified problems encountered in daily quality control according to standard operating procedures.	
25.0	Demonstrate knowledge of the operation of computer systems. The student will be able to: 	
25.01	Discuss the role of computer systems in laboratory data management.	
25.02	Demonstrate knowledge of common computer terminology.	
25.03	Demonstrate entry-level computer operations for specimen accessioning, data reporting, and quality control recording.	
25.04	Demonstrate entry-level operational skills in the use of computer-interfaced analytical instrumentation.	
26.0	Demonstrate an understanding of the basic principles of molecular diagnostics. The student will be able to:	
26.01	Discuss the principles and major steps of the polymerase chain reaction (PCR).	
26.02	Discuss the structure, function, and components of DNA and RNA.	
26.03	Define the key terms of molecular diagnostics.	
26.04	List and describe the principles of DNA, RNA, and molecular diagnostic testing.	
26.05	Compare the advantages and disadvantages of molecular techniques over traditional diagnostic tests for infectious diseases.	
26.06	Identify the types of samples appropriate for molecular diagnostics.	
26.07	Discuss the ethical impact of genetic technologies. 	
26.08	Outline requirements for reducing contamination in a molecular lab.	
26.09	Discuss nucleic acid probes and their role in clinical laboratory diagnostics.	
27.0	Demonstrate knowledge of urinalysis and body fluids principles and procedures. The student will be able to:	
27.01	Identify the components of the urinary system and explain their functions. 	
27.02	Discuss diseases affecting the urinary system.	
27.03	Describe collection, transport, and storage procedures for random and timed urine specimens.	
27.04	Discuss specific gravity techniques.	
27.05	Perform non-automated urinalysis techniques for chemical exam of the urine and interpret results. 	
27.06	Demonstrate the proper use of automated urinalysis analyzers.	
27.07	Describe renal function tests.	
27.08	Describe principles of and perform routine physical and chemical analyses on urine.	
27.09	Prepare urine sediments and perform identification and quantitation of microscopic formed elements. 	
27.10	Correlate abnormal physical, chemical, and microscopic urine results with associated pathological conditions.	
27.11	Differentiate between transudates and exudates. 	
27.12	Perform physical, chemical, and microscopic evaluations of common body fluids. 	
28.0	Demonstrate knowledge of hematological principles and procedures. The student will be able to:	
28.01	Discuss the organs, cells, and cellular interactions of the lymphoid, myeloid, and reticuloendothelial systems.	
28.02	Demonstrate an understanding of basic concepts of hematopoiesis. 	
28.03	Identify the components of blood.	
28.04	Discuss the function of formed elements of blood.	
28.05	Demonstrate an understanding of the synthesis of normal and abnormal molecular structure of hemoglobin, common hemoglobinopathies, and associated tests.	
28.06	Describe normal hemoglobin-oxygen function using the oxygen dissociation curve (ODC).	
28.07	Discuss assessment and impact of preanalytical, analytical, and post-analytical factors on hematology testing.	
28.08	Calculate red blood cell indices.	
28.09	Discuss selected cytochemical staining and flow cytometry procedures.	
28.10	Evaluate red blood cell morphology.	
28.11	State the review process of histogram/scatterplot/scattergram analysis. 	
28.12	Describe the categories used in a morphological classification of anemias.	
28.13	Correlate complete blood cell results with a peripheral exam of blood smear.	
28.14	List the white blood cell maturation sequence and identify distinguishing morphology for the stages of developing white blood cells. 	
28.15	Discuss normal and abnormal hematology findings, reference ranges, and associated diseases.	
28.16	Demonstrate an understanding of normal and abnormal white cell morphology, related disease states, and associated tests.	
28.17	Discuss the principles of and perform routine hematology procedures, applying quality control procedures as necessary.	
28.18	Perform commonly used methods to evaluate leukocytes, correlate, and verify automated cell counts with established criteria.	
28.19	Identify characteristic findings of nonmalignant leukocytic disorders (e.g., shift to the left, toxic granulation, Döhle bodies, etc.). 	
28.20	Perform techniques of manual blood smear evaluation, including white blood cell differential, red cell, and platelet morphology.	
28.21	Correlate peripheral blood evaluation with automated cell analysis.	
28.22	Perform platelet and white blood cell counts on specimens using manual techniques, diluents, and correlate counts with peripheral smear.	
29.0	Demonstrate knowledge of hemostasis and related diagnostic principles and procedures. The student will be able to:	
29.01	Discuss and define the mechanisms of hemostasis, including bleeding and clotting.	
29.02	Discuss common coagulopathies and associated treatments. 	
29.03	Discuss the assessment and impact of pre-analytical factors on hemostasis testing	
29.04	Describe the principles of and perform routine testing used in the evaluation of primary and secondary hemostasis. 	
29.05	Demonstrate use of hemostasis laboratory equipment.	
29.06	Discuss additional hemostasis tests performed to differentiate the cause of abnormal routine tests. 	
30.0	Demonstrate knowledge of immunology principles and procedures. The student will be able to:	
30.01	Discuss the functions of the cells of the immune system, cytokines, and regulatory molecules.	
30.02	Discuss physical and chemical properties of immunogens (antigens), immunoglobulins (antibodies), and complement.	
30.03	Compare and contrast the principles of basic agglutination, flocculation, and precipitation procedures in immunology/serology.	
30.04	Perform basic procedures in immunology/serology.	
30.05	Discuss principles of serum protein electrophoresis and immunofixation. 	
30.06	Discuss the clinical significance of the commonly performed immunological tests.	
30.07	Discuss selected serological tests.	
31.0	Demonstrate knowledge of clinical chemistry principles and procedures. The student will be able to:	
31.01	Identify the chemistry analytes used to evaluate various organ functions. 	
31.02	Discuss principles of and perform common renal function tests.	
31.03	Discuss carbohydrate, protein, and lipid metabolism.	
31.04	Discuss principles of and perform commonly ordered tests related to carbohydrate, protein, and lipid metabolism.	
31.05	Discuss the liver and its functions and related analytes.	
31.06	Discuss principles of and perform commonly ordered liver function tests.	
31.07	Discuss enzyme classification, origin, activity, and function.	
31.08	Discuss principles of and perform commonly ordered enzyme procedures.	
31.09	Discuss electrolyte balance as related to health and disease.	
31.10	Discuss principles of and perform electrolyte analyses.	
31.11	Discuss principles of and perform commonly ordered tests to evaluate cardiac function.	
31.12	Discuss the physiology of the endocrine system and the principal tests used to evaluate endocrine function.	
31.13	Discuss the role of the laboratory in therapeutic drug monitoring and toxicology.	
31.14	Discuss and perform general electrophoresis techniques.	
31.15	Discuss the clinical significance of commonly ordered clinical chemistry tests.	
31.16	Demonstrate knowledge of principles of instrumentation as related to the clinical chemistry laboratory.	
31.17	Discuss techniques of clinical chemistry related to the standardization of procedures and use of standards, blanks, and controls. 	
31.18	Discuss other techniques of clinical chemistry.	
31.19	Discuss basic techniques of clinical chemistry related to normal and abnormal physiology. 	
32.0	Demonstrate knowledge of immunohematology principles and procedures. The student will be able to:	
32.01	Discuss donor interviews, criteria for selection, phlebotomy preparation, and donor blood processing. 	
32.02	Discuss blood component collection, preparation, storage, and use.	
32.03	Describe the roles of the Federal Drug Administration (FDA), American Association of Blood Banks (AABB), and state agencies, and how to contact each.	
32.04	Compare the advantages and disadvantages of autologous versus homologous (allogenic) blood collection and transfusion.	
32.05	Discuss the basic genetics of the blood group antigens.	
32.06	Discuss the ABO blood group system testing procedures and recognize ABO discrepancies. 	
32.07	Describe required tests on recipient blood samples. 	
32.08	Discuss and differentiate other blood group systems such as Duffy, Kell, Kidd, S,s, Lu, and the common cold-reacting antibodies such as Le, P, I, M, and N.	
32.09	Perform Rh testing to determine Rh phenotypes.	
32.10	Perform and interpret antibody screening.  	
32.11	Perform antibody identification tests to detect clinically significant antibodies.  	
32.12	Discuss the safety and determine the compatibility of blood components for transfusion.	
32.13	Discuss and perform routine compatibility testing, including the immediate spin crossmatch and the electronic crossmatch.	
32.14	Discuss and perform phenotyping on recipient and donor specimens.	
32.15	Identify symptoms of the suspected transfusion reaction and the required laboratory work-up.	
32.16	Discuss immune hemolytic disorders and perform the direct antiglobulin test. 	
32.17	Discuss specialized techniques in immunohematology.	
32.18	Perform quality control (QC) on reagents.	
32.19	Describe the pathophysiology of hemolytic disease of the fetus and newborn.	
33.0	Demonstrate knowledge of microbiological principles and procedures. The student will be able to:	
33.01	Discuss microbial taxonomy and nomenclature.	
33.02	Discuss bacterial metabolism, reproduction, cell structures, and their functions.	
33.03	Discuss classification, composition, and preparation of culture media.	
33.04	Discuss the human pathogenesis of bacteria.	
33.05	Discuss and perform sterilization techniques. 	
33.06	Perform culturing techniques for urine, stool, wound, respiratory, body fluids, and blood specimens. 	
33.07	Perform techniques of microbiology related to inoculation and transfer of cultures. 	
33.08	Discuss the principles of Gram and Acid-Fast Bacilli (AFB) stains.	
33.09	Accurately perform, read, and report Gram stains.	
33.10	Perform techniques necessary for isolation and identification of aerobic, anaerobic, and facultative bacterial organisms. 	
33.11	Identify commonly encountered aerobic bacteria through morphological, physical, and biochemical properties.	
33.12	Perform and interpret antimicrobial susceptibility tests.	
33.13	Discuss the collection and handling of specimens for fungal, mycobacterial, and viral culture. 	
33.14	Prepare and examine specimens and identify ova and parasites when present.	
34.0	Demonstrate knowledge of advanced hematological principles and procedures. The student will be able to:	
34.01	Correlate histogram/scatterplot/scattergram with differential results. 	
34.02	Describe the categories used in the etiological classification of anemias.	
34.03	Perform quality control procedures and identify preestablished quality control limits/criteria. 	
34.04	Perform critical analysis using preestablished reporting guidelines, identification, classification, and differentiation of neoplastic disorders.	
34.05	Discuss the principles and applications of flow cytometry.	
34.06	Discuss the principle of hemoglobin electrophoresis with pattern interpretation.	
35.0	Demonstrate knowledge of advanced hemostasis testing. The student will be able to:	
35.01	Discuss the principle of mixing studies and factor assays. 	
35.02	Correlate the laboratory test results for fibrinolysis with conditions affecting the fibrinolytic system.	
35.03	Perform additional hemostasis tests to differentiate the cause of abnormal routine tests.	
35.04	Correlate laboratory results with possible inherited and/or acquired coagulation abnormalities.	
35.05	Discuss and perform platelet studies (e.g., platelet aggregation, thromboelastography (TEG), etc.).	
35.06	Discuss and perform testing to monitor antithrombotic drug therapies.	
35.07	Describe methods available for molecular testing and the clinical hemostasis lab.	
36.0	Demonstrate knowledge of advanced microbiological principles and procedures. The student will be able to:	
36.01	Discuss procedures for aerobic, facultative, and anaerobic identification, and the clinical significance of different bacteria.	
36.02	Classify fungi and state their clinical significance.	
36.03	Identify pathogenic fungi and perform techniques used in identifying fungi.	
36.04	Differentiate between pathogenic and nonpathogenic fungi.	
36.05	Discuss and perform automated microbiological procedures.	
36.06	Identify life cycles, modes of transmission, prevention, and pathophysiology of clinically significant parasites.	
36.07	Discuss microbial and immunological techniques to identify clinically significant viruses.	
36.08	Discuss classification and related disease states of clinically significant viruses.	
36.09	Identify commonly used antibiotics, their usage, and mechanisms of activity.	
36.10	Discuss and perform molecular testing to identify bacteria, parasites, fungi, and significant viruses.	
37.0	Demonstrate knowledge of advanced clinical chemistry principles and procedures. The student will be able to:	
37.01	Perform, calculate, analyze, and recognize normal/abnormal electrophoresis procedures.	
37.02	Perform and interpret immunoassay procedures and results.	
37.03	Perform and recognize associated disease states for selected isoenzyme assays.	
37.04	Perform, calculate, and recognize associated disease states for blood lipid profiles.	
37.05	Perform selected procedures related to analyzing hormones and evaluating endocrine function.	
37.06	Perform selected assays for therapeutic drug monitoring and toxic substances.	
37.07	Analyze toxicology results. 	
37.08	Discuss the principles and procedures of blood gas analysis, including arterial specimen collection and clinical significance.	
38.0	Demonstrate knowledge of advanced immunological procedures. The student will be able to:	
38.01	Perform serological tests such as immunofluorescence assays.	
38.02	Discuss and interpret antinuclear antibody patterns and their relationship to disease states.	
38.03	Discuss MHC and HLA proteins and their application to transplant technology and rejection.	
39.0	Demonstrate knowledge of advanced immunohematology principles and procedures. The student will be able to:	
39.01	Describe donor suitability criteria.	
39.02	Discuss the various parameters of the required testing of donor blood according to AABB Standards.	
39.03	Describe the preparation and use of washed red cells, leukocyte-reduced red cells, and platelet concentrations from donor units.	
39.04	Describe the preparation and use of cryoprecipitate and fresh frozen plasma.	
39.05	Describe the principle and use of the antiglobulin test, both direct and indirect methods, including the purpose of using IgG-sensitized cells.	
39.06	Perform methods of adsorption and elution techniques.	
39.07	Perform procedures for identification of multiple antibodies in the recipient specimen, including enzyme enhancement, dosage, temperature, and complement binding.	
39.08	Determine the appropriate dosage of Rh Immune Globulin based on test results.  	
39.09	Interpret the preliminary investigation results to determine whether a transfusion reaction has occurred.	
40.0	Demonstrate an understanding of advanced principles of molecular diagnostics. The student will be able to:	
40.01	Describe the steps used to prepare genomic DNA and RNA from blood and buccal cells.	
40.02	Discuss the extraction, purification, and storage of nucleic acids.	
40.03	Describe the process of Fluorescent In-Situ Hybridization (FISH).	
40.04	Compare and contrast real-time PCR and conventional PCR.	
40.05	Explain baseline threshold and CT-values of PCR curves. 	
40.06	Explain Sanger sequencing. 	
40.07	Explain Next-generation sequencing.	

Additional Information

Laboratory Activities

Laboratory investigations that include scientific inquiry, research, measurement, problem solving, emerging technologies, tools, and equipment, as well as experimental, quality, and safety procedures, are an integral part of this career and technical program/course. Laboratory investigations benefit all students by developing an understanding of the complexity and ambiguity of empirical work, as well as the skills required to manage, operate, calibrate, and troubleshoot equipment/tools used to make observations. Students understand measurement error and have the skills to aggregate, interpret, and present the resulting data. Equipment and supplies should be provided to enhance hands-on experiences for students.

Clinical learning experiences in a clinical laboratory and related areas are an integral part of this program.  Clinical learning experiences should reflect the full breadth of responsibilities expected of a Medical Laboratory Technician and should include appropriate experience in each of the areas of the laboratory described herein.  The specified length for each of the courses listed is inclusive of clinical experience for each of the respective laboratory sections.

Special Notes

This program meets the Department of Health HIV/AIDS, Domestic Violence, and Prevention of Medical Errors education requirements.  Upon completion of this program, the instructor will provide a certificate to the student verifying that these requirements have been met.

If students in this program are seeking a licensure, certificate, or registration through the Department of Health, please refer to 456.0635, F.S. for more information on disqualification for a license, certificate, or registration through the Department of Health. 

The Core should be taken first in the program.  Following the successful completion of the core, the student is eligible to take the National Health Care Foundation Skill Standards Assessment with instructor approval and the completion of a portfolio.

General Education Course Requirements for AS and AAS Degrees

State Board of Education Rule 6A-14.030(4), F.A.C., identifies 15 credit hours as the minimum amount of general education coursework required in the Associate of Science (AS) degree and the Associate of Applied Science (AAS) degree.  In addition, Rule 6A-14.0303, F.A.C., implements s. 1007.25, F.S., and requires students entering a technical education degree program in the 2022-2023 academic year, and thereafter, to complete at least one identified core course in each subject area as part of the general education course requirements (15 credit hours total) before a degree is awarded. The core subject areas include: 
•	Communication. 
•	Humanities.
•	Mathematics. 
•	Natural Sciences.
•	Social Sciences.

Career and Technical Student Organization (CTSO)

CTSOs are co-curricular career and technical student organizations providing leadership training and reinforcing specific career and technical skills. Career and Technical Student Organizations provide activities for students as an integral part of the instruction offered. Other CTSOs not listed in this curriculum framework or recognized by the Florida Department of Education are permissible provided they support student mastery over the standards and benchmarks of this curriculum framework.

Accommodations

Federal and state legislation requires the provision of accommodations for students with disabilities to meet individual needs and ensure equal access. Postsecondary students with disabilities must self-identify, present documentation, request accommodations if needed, and develop a plan with their counselor and/or instructors. Accommodations received in postsecondary education may differ from those received in secondary education. Accommodations change the way the student is instructed. Students with disabilities may need accommodations in such areas as instructional methods and materials, assignments and assessments, time demands and schedules, learning environment, assistive technology, and special communication systems. Documentation of the accommodations requested and provided should be maintained in a confidential file.