Florida Department of Education
Curriculum Framework 

Program Title:	Medical Laboratory Technology
Career Cluster:	Health Science

AS	
CIP Number	1351100405	
Program Type	College Credit	
Program Length	76 credit hours	
CTSO	HOSA	
SOC Codes (all applicable) 	Please see the CIP to SOC Crosswalk located at the link below.                            	
CTE Program Resources 	http://www.fldoe.org/academics/career-adult-edu/career-tech-edu/program-resources.stml 	

Purpose

This program offers a sequence of courses that provides coherent and rigorous content aligned with challenging academic standards and relevant technical knowledge and skills needed to prepare for further education and careers in the Health Science career cluster; provides technical skill proficiency, and includes competency-based applied learning that contributes to the academic knowledge, higher-order reasoning and problem-solving skills, work attitudes, general employability skills, technical skills, and occupation-specific skills, and knowledge of all aspects of Health Science career cluster. 

The purpose of this program is to prepare students for employment as medical/clinical laboratory technologist (medical clinical laboratory technologist) or medical laboratory technologists (associate degree) or to provide supplemental training for persons previously or currently employed in these occupations.

The content includes but is not limited to specific techniques and instruments, identification of factors directly affecting procedures and results, confirmation of results and monitoring quality control programs within pre-determined parameters, and correction of errors using pre-set standards.  A clinical component is a necessary element of this program.

Additional Information relevant to this Career and Technical Education (CTE) program is provided at the end of this document.

Program Structure
This program is a planned sequence of instruction consisting of 76 credit hours.

Regulated Programs
This program is regulated by the Florida Board of Clinical Laboratory Personnel: http://floridasclinicallabs.gov/

Standards

After successfully completing this program, the student will be able to perform the following:

01.0	Demonstrate knowledge of the healthcare delivery system and health occupations.
02.0	Demonstrate the ability to communicate and use interpersonal skills effectively.
03.0	Demonstrate legal and ethical responsibilities.
04.0	Demonstrate an understanding of and apply wellness and disease concepts.
05.0	Recognize and practice safety and security procedures.
06.0	Recognize and respond to emergency situations.
07.0	Recognize and practice infection control procedures.
08.0	Demonstrate an understanding of information technology applications in healthcare.
09.0	Demonstrate employability skills.
10.0	Demonstrate knowledge of blood borne diseases, including HIV/AIDS.
11.0	Apply basic math and science skills. 
12.0	Demonstrate accepted professional, communication and interpersonal skills.
13.0	Discuss phlebotomy in relation to the health care setting.
14.0	Identify the anatomic structure and function of body systems in relation to services performed by phlebotomist.
15.0	Recognize and identify collection reagents supplies, equipment and interfering chemical substances.
16.0	Demonstrate skills and knowledge necessary to perform phlebotomy.
17.0	Practice infection control following standard precautions.
18.0	Practice accepted procedures of transporting, accessioning and processing specimens.
19.0	Practice quality assurance and safety.
20.0	Demonstrate knowledge and use of basic laboratory equipment and techniques.
21.0	Demonstrate basic knowledge of and perform clinical laboratory Point of Care (POC) testing (Waived).
22.0	Demonstrate basic knowledge of and perform Point of Care (POC) Testing using CLIA approved Waived instrumentation. 
23.0	Discuss the general responsibilities and functions encountered by a medical technician.
24.0	Apply quality assurance principles and safety protocols.
25.0	Demonstrate knowledge of the operation of computer systems.
26.0	Demonstrate an understanding of the basic principles of molecular diagnostics.
27.0	Demonstrate knowledge of urinalysis and body fluids principles and procedures.
28.0	Demonstrate knowledge of hematological principles and procedures.
29.0	Demonstrate knowledge of hemostasis and related diagnostic principles and procedures.
30.0	Demonstrate knowledge of immunology principles and procedures.
31.0	Demonstrate knowledge of clinical chemistry principles and procedures.
32.0	Demonstrate knowledge of immunohematology principles and procedures.
33.0	Demonstrate knowledge of microbiological principles and procedures.
34.0	Demonstrate knowledge of advanced hematological principles and procedures.
35.0	Demonstrate knowledge of advanced hemostasis testing. 
36.0	Demonstrate knowledge of advanced microbiological principles and procedures.
37.0	Demonstrate knowledge of advanced clinical chemistry principles and procedures.
38.0	Demonstrate knowledge of advanced immunological procedures.
39.0	Demonstrate knowledge of advanced immunohematology principles and procedures.
40.0	Demonstrate and understanding of advanced principles of molecular diagnostics.

Florida Department of Education
Student Performance Standards

Program Title:	Medical Laboratory Technology 
CIP Number:		1351100405  
Program Length:	76 credit hours

Standards 1-11 are referred to as the Health Science Core and are required standards in this program. Secondary and Postsecondary students completing the health science core will not have to repeat the core in any other health science program in which it is a part.  When the recommended sequence is followed, the structure allows students to complete at specified points for employment or remain for advanced training or cross-training.  

To ensure consistency whenever these courses are offered, the health science core standards (1-11) and benchmarks have been placed in a separate document. To access this document, visit this link: 
https://www.fldoe.org/core/fileparse.php/20569/urlt/health-sci-core-psav-cc-2324.rtf

At the completion of this program, the student will be able to:	
12.0	Demonstrate accepted professional, communication, and interpersonal skills.  The student will be able to:	
12.01	Demonstrate the appropriate professional behavior of a phlebotomist.	
12.02	Explain to the patient the procedure to be used in specimen collection. 	
12.03	Explain in detail the importance of identifying patients correctly when drawing blood.	
12.04	Describe the scope of practice (job skills and duties) for a phlebotomist.	
12.05	List and describe professional organizations that provide accreditation for phlebotomy programs and provide certification for phlebotomists. 	
12.06	Explain the importance of continuing education in relation to certification to maintain competency and skills.	
13.0	Discuss phlebotomy in relation to the health care setting.  The student will be able to:	
13.01	List, classify and discuss various departments and services within the health care setting in which the phlebotomist must interact with to obtain laboratory specimens from patients.	
13.02	Identify the major departments/sections with the clinical laboratory, the major types of procedures run in each department/section, and their specimen requirements.	
13.03	Describe roles of the major classifications of clinical laboratory personnel (i.e., pathologist, chief/administrative technologist, CLS, MLS, MLT, MT, phlebotomist, lab assistant, etc.).	
14.0	Identify the anatomic structure and function of body systems in relation to services performed by phlebotomist.  The student will be able to:	
14.01	Describe and define major body systems with emphasis on the circulatory system.	
14.02	List and describe the main superficial veins used in performing venipuncture.	
14.03	 Locate the most appropriate sites(s) for capillary and venipuncture.	
14.04	Describe the function of the following blood components: erythrocytes, thrombocytes, leukocytes, and plasma.	
14.05	Compare and contrast between serum and plasma as it relates to blood collection.	
14.06	Discuss hemostasis as it relates to blood collection.	
15.0	Recognize and identify collection reagents supplies, equipment, and interfering chemical substances.  The student will be able to:	
15.01	Identify and discuss proper use of appropriate types of equipment needed to collect various clinical laboratory blood specimens by venipuncture.	
15.02	Explain the special precautions and types of equipment needed to collect blood from the pediatric patient. 	
15.03	Identify and discuss proper use of supplies used in collecting micro-specimens.	
15.04	Identify and discuss the proper use of the various types of anticoagulants, preservatives and gels used in blood collection and the vacuum tube color-codes for these additives.	
15.05	Describe the types of patient's specimens that are analyzed in the clinical laboratory and the phlebotomist's role in collecting and/or transporting these specimens to the laboratory.	
15.06	Describe substances potentially encountered during phlebotomy which can interfere in analysis of blood constituents.	
15.07	Define and utilize correct medical terminology and metric measurement needed for specimen collection.	
16.0	Demonstrate skills and knowledge necessary to perform phlebotomy. – The student will be able to:	
16.01	Follow approved procedure for completing a laboratory requisition form.	
16.02	Recognize a properly completed requisition and apply established protocol for patient and specimen identification for transport to a reference lab.	
16.03	Demonstrate knowledge of established protocol for patient and specimen identification.	
16.04	Discuss appropriate methods for facilitating and preparing the patient for capillary and venipuncture collection.	
16.05	List appropriate antiseptic agents useful in preparing sites for capillary and venipuncture.	
16.06	Know how to perform venipuncture by evacuated tube, butterfly and syringe systems.	
16.07	Describe the correct order of draw according to CLSI guidelines. 	
16.08	Describe the use of barcoding systems used for positive patient identification and specimen identification.  	
16.09	 Convey an understanding of capillary puncture using appropriate supplies and techniques for both adults and pediatric patients.	
16.10	Describe the most common complications associated with capillary and venipuncture, their causes, prevention, and treatment.	
16.11	Recognize and respond to possible adverse patient reactions such as allergies, convulsions, syncope, and light headedness.	
16.12	Perform appropriate procedures for disposing of used or contaminated capillary and venipuncture supplies.	
16.13	Perform appropriate techniques for making a peripheral blood smear for hematologic evaluation.	
16.14	Demonstrate the proper procedure for collecting blood cultures.	
16.15	Discuss the effects of hemolysis and methods of prevention. 	
16.16	Demonstrate a working understanding of how age and weight of patients impacts the maximum amount of blood that can be safely drawn.	
17.0	Practice infection control following standard precautions.  The student will be able to:	
17.01	Define the term "hospital acquired infection”.	
17.02	Demonstrate proper hand hygiene. 	
17.03	Comply with universal/standard precautions. 	
17.04	Identify potential routes of infection and their complications.	
18.0	Practice accepted procedures of transporting, accessioning, and processing specimens.  The student will be able to:	
18.01	Follow the approved procedure for preparation and processing (e.g., centrifugation, separation, aliquoting, labeling, and storage) of serum, plasma, urine, sputum, stool, and wound culture specimens.  	
18.02	Demonstrate knowledge of accessioning procedures.	
18.03	Describe the significance of time constraints for specimen collection, transporting, and delivery.	
18.04	Describe routine procedures for transporting and processing specimens including DOT packaging requirements.	
19.0	Practice quality assurance and safety.  The student will be able to:	
19.01	Distinguish and perform procedures which ensure reliability of test results when collecting blood specimens.	
19.02	Demonstrate knowledge of and practice appropriate patient safety.	
19.03	Practice safety in accordance with OSHA (State & Federal guidelines) for chemical, biological, and PPE established procedures including proper disposal of sharps.	
19.04	Follow documentation procedures for work related accidents.	
19.05	Understand Joint Commission patient safety goals and other accrediting/regulatory agency guidelines.	
20.0	Demonstrate knowledge and use of basic laboratory equipment and techniques.  The student will be able to:	
20.01	Identify the parts of the microscope and explain the function of each.	
20.02	Demonstrate the proper technique for operation of the microscope.	
20.03	Demonstrate use of standard laboratory equipment including glassware, pipettes, and centrifuge.	
20.04	Perform basic laboratory math calculations. 	
20.05	Understand the principles of quality assurance to correct problems encountered in monitoring daily quality control.	
20.06	Evaluate laboratory findings to confirm results according to standard operating procedure.	
20.07	Demonstrate knowledge of principles and operation of laboratory instruments.	
21.0	Demonstrate the basic knowledge of and perform clinical laboratory Point of Care (POC) testing (Waived).  The student will be able to:	
21.01	Demonstrate the ability to interpret instructions of point of care testing including, but not limited to the following:   
21.01.01	Test principle
21.01.02	Storage and Stability
21.01.03	Internal vs. External Quality Control 
21.01.04	Specimen collection and preparation 
21.01.05	Directions for use 
21.01.06	Interpretation of results 
21.01.07	Interfering substances	
21.02	Demonstrate and discuss knowledge of lot numbers use and importance in regard to both kits and reagents.	
21.03	Demonstrate knowledge of the frequency in which quality control procedures should be performed.	
21.04	Explain the CLIA 88 classification of laboratory testing into waived, moderate, and highly complex including the personnel qualified to perform each. 	
22.0	Demonstrate basic knowledge of and perform point of care (POC) testing using CLIA approved waived instrumentation.  The student will be able to:	
22.01	Demonstrate and perform POC testing specific to microbiology, hematology, urinalysis, and clinical chemistry.	
22.02	Perform instrument maintenance.	
22.03	Demonstrate knowledge of quality control and calibrations involved within the POC instruments.	
22.04	Identify normal limits and associate abnormal results with disease or disorders.	
22.05	Discuss the significance of reporting critical values as it applies to point of care testing.	
23.0	Discuss the general responsibilities and functions encountered by a medical technician.  The students will be able to: 	
23.01	Ask appropriate scientific questions and recognize what is involved in experimental approaches to the solutions of such questions.	
23.02	Communicate laboratory results to healthcare professionals. 	
23.03	Demonstrate ability to evaluate laboratory results.  	
23.04	Demonstrate ability to report laboratory results in written or oral form.	
23.05	Discuss the licensure and certification requirements of the major classifications of clinical laboratory personnel.	
24.0	Apply quality assurance principles and safety protocols.  The student will be able to: 	
24.01	Assess specimen acceptability using standard operating procedure including rejection/recollection criteria. 	
24.02	Describe procedures for transporting and processing specimens.	
24.03	Describe clinical laboratory role in providing quality assurance in laboratory testing, reporting, and the use and maintenance of equipment.	
24.04	Understand the need for calibration of laboratory equipment. 	
24.05	Demonstrate and record quality control procedures required for the tests performed and recognize unacceptable results.	
24.06	Report identified problems encountered in daily quality control according to standard operating procedures.	
24.07	Comply with current OSHA regulations regarding laboratory hazards.	
25.0	Demonstrate knowledge of the operation of computer systems.  The student will be able to: 	
25.01	Discuss the role of computer systems in laboratory data management.	
25.02	Demonstrate knowledge of common computer terminology.	
25.03	Demonstrate entry level computer operations for specimen accessioning, data reporting, and quality control recording.	
25.04	Demonstrate entry level operational skills in the use of computer-interfaced analytical instrumentation.	
26.0	Demonstrate an understanding of the basic principles of molecular diagnostics. - The student will be able to:	
26.01	Discuss the principles and major steps of the polymerase chain reaction (PCR).	
26.02	Label the organelles and important parts of a eukaryotic animal cell.	
26.03	Describe the function of the organelles and important parts of a eukaryotic animal cell.	
26.04	Discuss the structure, function, and components of DNA and RNA.	
26.05	Define the key terms of molecular diagnostics.	
26.06	Understand the principles of molecular diagnostic testing.	
26.07	Compare the advantages and disadvantages of molecular techniques over traditional diagnostic tests for infectious diseases.	
26.08	List molecular tests associated with the identification of microorganisms.	
26.09	Identify the types of samples appropriate for molecular diagnostics.	
26.10	Discuss the ethical impact of genetic technologies. 	
26.11	Outline requirements for reducing contamination in a molecular lab.	
26.12	Discuss nucleic acid probes and their role in clinical laboratory diagnostics.	
27.0	Demonstrate knowledge of urinalysis and body fluids principles and procedures.  The student will be able to:	
27.01	Identify the components of the urinary system and explain their functions. 	
27.02	Discuss diseases affecting the urinary system.	
27.03	Describe collection, transport and storage procedures for random and timed urine specimens.	
27.04	Discuss specific gravity techniques; calibration and use of the refractometer.	
27.05	Perform dipstick or tablet (non-automated) urinalysis techniques for chemical exam of the urine and interpret results 	
27.06	Demonstrate the proper use of automated urinalysis analyzers.	
27.07	Describe renal function tests.	
27.08	Describe principles of and perform routine physical and chemical analyses on urine.	
27.09	Prepare urine sediments and perform identification and quantitation of microscopic formed elements. 	
27.10	Correlate abnormal physical, chemical, and microscopic urine results with associated pathological conditions.	
27.11	Differentiate between transudates and exudates. 	
27.12	Discuss miscellaneous body fluids to include cerebral spinal, serous, seminal, and joint fluids. 	
27.13	Perform physical, chemical, and microscopic evaluations of common body fluids. 	
28.0	Demonstrate knowledge of hematological principles and procedures.  The student will be able to:	
28.01	Discuss the organs, cells, and cellular interaction of the lymphoid, myeloid, and reticuloendothelial systems.	
28.02	Demonstrate an understanding of basic concepts of hematopoiesis. 	
28.03	Identify the components of blood.	
28.04	Discuss the function of formed elements of blood.	
28.05	Demonstrate an understanding of the synthesis of normal and abnormal molecular structure of hemoglobin, common hemoglobinopathies, and associated tests.	
28.06	Describe normal hemoglobin-oxygen function using the oxygen dissociation curve (ODC).	
28.07	Discuss assessment and impact of preanalytical, analytical, and post-analytical factors on hematology testing.	
28.08	Calculate red blood cell indices.	
28.09	Discuss selected cytochemical staining and flow cytometry procedures.	
28.10	Evaluate red blood cell morphology.	
28.11	State the review process of histogram/scatterplot/scatter gram analysis. 	
28.12	Describe the categories used in a morphological classification of anemias.	
28.13	Correlate complete blood cell results with peripheral exam of blood smear.	
28.14	List the white blood cell maturation sequence and identify distinguishing morphology for stages of developing white blood cells. 	
28.15	Discuss normal and abnormal hematology findings, reference ranges, and associated diseases.	
28.16	Demonstrate an understanding of normal and abnormal white cell morphology, related disease states and associated tests.	
28.17	Discuss the principles of and perform routine hematology procedures applying quality control procedures, as necessary.	
28.18	Perform commonly used methods to evaluate leukocytes, correlate and verify automated cell counts with established criteria.	
28.19	Identify characteristic findings of nonmalignant leukocytic disorders, (e.g., shift to the left, toxic granulation, Döhle bodies, etc.). 	
28.20	Perform techniques of manual blood smear evaluation including white blood cell differential, red cell and platelet morphology.	
28.21	Correlate peripheral blood evaluation with automated cell analysis.	
28.22	Perform platelet counts on patient and control specimens using manual and automated techniques and correlate counts with peripheral smear.	
29.0	Demonstrate knowledge of hemostasis and related diagnostic principles and procedures.  The student will be able to:	
29.01	Discuss and define the mechanisms of hemostasis including bleeding and clotting.	
29.02	Discuss common coagulopathies and associated treatments. 	
29.03	Discuss assessment and impact of pre-analytical factors on hemostasis testing	
29.04	Describe the principles of and perform routine testing used in the evaluation of primary and secondary hemostasis. 	
29.05	Discuss additional hemostasis tests performed to differentiate the cause of abnormal routine tests. 	
30.0	Demonstrate knowledge of immunology principles and procedures.  The student will be able to:	
30.01	Discuss the functions of the cells of the immune system, cytokines, and regulatory molecules.	
30.02	Discuss physical and chemical properties of immunogens (antigens), immunoglobulins (antibodies), and complement 	
30.03	Compare and contrast the principles of basic agglutination, flocculation and precipitation procedures in immunology/serology.	
30.04	Perform basic procedures in immunology/serology.	
30.05	Discuss principles of serum protein electrophoresis and immunofixation. 	
30.06	Discuss the clinical significance of the commonly performed immunological tests.	
30.07	Discuss selected serological tests such as immunoassays.	
31.0	Demonstrate knowledge of clinical chemistry principles and procedures.  The student will be able to:	
31.01	Identify the chemistry analytes used to evaluate various organ function. 	
31.02	Discuss the renal system and related analytes.	
31.03	Discuss principles of, and perform common renal function tests.	
31.04	Discuss carbohydrate, protein, and lipid metabolism.	
31.05	Discuss principles of, and perform commonly ordered tests related to carbohydrate, protein, and lipid metabolism.	
31.06	Discuss the liver and its functions and related analytes.	
31.07	Discuss principles of and perform commonly ordered liver function tests.	
31.08	Discuss enzyme classification, origin, activity and function.	
31.09	Discuss principles of and perform commonly ordered enzyme procedures.	
31.10	Discuss electrolyte balance as related to health and disease.	
31.11	Discuss principles of and perform electrolyte analyses.	
31.12	Discuss principles of and perform commonly ordered tests to evaluate cardiac function.	
31.13	Discuss the physiology of the endocrine system and the principal tests used to evaluate endocrine function.	
31.14	Discuss the role of the laboratory in therapeutic drug monitoring and toxicology.	
31.15	Discuss and perform general electrophoresis techniques.	
31.16	Discuss the clinical significance of commonly ordered clinical chemistry tests.	
31.17	Demonstrate knowledge of principles of instrumentation as related to the clinical chemistry laboratory.	
31.18	Discuss techniques of clinical chemistry related to standardization of procedure and use of standards and controls. 	
31.19	Discuss other techniques of clinical chemistry.	
31.20	Discuss basic techniques of clinical chemistry related to normal and abnormal physiology. 	
32.0	Demonstrate knowledge of immunohematology principles and procedures.  The student will be able to:	
32.01	Discuss donor interview, criteria for selection, phlebotomy preparation, and donor blood processing. 	
32.02	Discuss blood component collection, preparation, storage, and use.	
32.03	Describe the roles of FDA, AABB, and state agencies and how to contact each.	
32.04	Compare advantages and disadvantages for autologous, versus homologous (allogenic) blood collection and transfusion.	
32.05	Discuss basic genetics of the blood group antigens 	
32.06	Discuss the ABO blood group system testing procedures and recognize ABO discrepancies. 	
32.07	Describe required tests on recipient blood samples. 	
32.08	Discuss and differentiate other blood group systems such as Duffy, Kell, Kidd, S,s, Lu, and the common cold-reacting antibodies such as Le, P, I, M, and N.	
32.09	Perform Rh testing to determine Rh phenotypes.	
32.10	Perform and interpret antibody screening.  	
32.11	Perform antibody identification tests to detect clinically significant antibodies.  	
32.12	Discuss the safety and determine compatibility of blood components for transfusion.	
32.13	Discuss and perform routine compatibility testing including the immediate spin crossmatch and the electronic crossmatch.	
32.14	Discuss and perform phenotyping on recipient and donor specimens.	
32.15	Identify symptoms of the suspected transfusion reaction and the required laboratory work-up.	
32.16	Discuss immune hemolytic disorders and perform the direct antiglobulin test. 	
32.17	Discuss specialized techniques.	
32.18	Perform quality control (QC) on reagents.	
32.19	Describe the pathophysiology of hemolytic disease of the fetus and newborn.	
33.0	Demonstrate knowledge of microbiological principles and procedures.  The student will be able to:	
33.01	Discuss microbial taxonomy and nomenclature.	
33.02	Discuss bacterial metabolism, reproduction, cell structures, and their functions.	
33.03	Discuss classification, composition, and preparation of culture media.	
33.04	Discuss the human pathogenesis of bacteria.	
33.05	Discuss and perform sterilization techniques. 	
33.06	Perform culturing techniques for urine, stool, wound, respiratory, body fluids, and blood specimens. 	
33.07	Perform techniques of microbiology related to inoculation and transfer of cultures. 	
33.08	Discuss the principles of Gram and AFB stains.	
33.09	Accurately perform, read, and report gram stains.	
33.10	Perform techniques necessary for isolation and identification of aerobic and anaerobic bacterial organisms. 	
33.11	Identify commonly encountered aerobic bacteria through morphological, physical, and biochemical properties.	
33.12	Perform and interpret antimicrobial susceptibility tests.	
33.13	Discuss collection and handling of specimens for fungal, mycobacterial, and viral culture. 	
33.14	Prepare and examine specimens, and identify ova and parasites when present.	
34.0	Demonstrate knowledge of advanced hematological principles and procedures.  The student will be able to:	
34.01	Correlate histogram/scatterplot/scatter gram with differential results.	
34.02	Describe the categories used in etiological classification of anemias.	
34.03	Perform procedures, apply appropriate quality control procedures, recognize and follow up, within pre-established reporting guidelines, laboratory procedures used in the identification, classification, and differentiation of neoplastic disorders. 	
34.04	Discuss the basic principles and applications of flow cytometry.	
34.05	Discuss the principle of hemoglobin electrophoresis with pattern interpretation.	
35.0	Demonstrate knowledge of advanced hemostasis testing.  The student will be able to:	
35.01	Discuss the principle of mixing studies and factor assays. 	
35.02	Correlate the laboratory test results for fibrinolysis with conditions affecting the fibrinolytic system.	
35.03	Perform additional hemostasis tests performed to differentiate the cause of abnormal routine tests.	
35.04	Correlate laboratory results with possible inherited and/or acquired coagulation abnormalities.	
36.0	Demonstrate knowledge of advanced microbiological principles and procedures.  The student will be able to:	
36.01	Discuss procedures for anaerobic identification and clinical significance of anaerobes.	
36.02	Classify fungi and state their clinical significance.	
36.03	Perform techniques used in identifying fungi.	
36.04	Identify pathogenic fungi.	
36.05	Discuss and perform automated microbiological procedures.	
36.06	Identify life cycles, modes of transmission, prevention and pathophysiology, of clinically significant parasites.	
36.07	Discuss microbial and immunological techniques to identify clinically significant viruses.	
36.08	Discuss classification and related disease states of clinically significant viruses.	
36.09	Identify commonly used antibiotics, their usage and mechanisms of activity.	
37.0	Demonstrate knowledge of advanced clinical chemistry principles and procedures.  The student will be able to:	
37.01	Perform, calculate, analyze, and recognize normal/abnormal electrophoresis procedures.	
37.02	Perform immunoassay procedures.	
37.03	Perform and recognize associated disease states for selected isoenzyme assays.	
37.04	Perform, calculate, and recognize associated disease states for blood lipid profiles.	
37.05	Perform selected procedures related to endocrine function.	
37.06	Perform selected assays for therapeutic and toxic substances.,	
37.07	Discuss the principles and procedures of blood gas analysis including arterial specimen collection and clinical significance.	
38.0	Demonstrate knowledge of advanced immunological procedures.  The student will be able to:	
38.01	Perform serological tests such as immunofluorescence assays.	
38.02	Discuss and interpret antinuclear antibody patterns and their relationship to disease states.	
38.03	Discuss MHC and HLA proteins and the application to transplant technology and rejection.	
39.0	Demonstrate knowledge of advanced immunohematology principles and procedures.  The student will be able to:	
39.01	Understand questions regarding donor suitability.	
39.02	Discuss the various parameters of required testing of donor blood according to AABB Standards.	
39.03	Describe the preparation and use of washed red cells, leukocyte-reduced red cells and platelet concentrations from donor units.	
39.04	Describe the preparation and use of cryoprecipitate and fresh frozen plasma.	
39.05	Describe the principle and use of the antiglobulin test, both direct and indirect methods, including the purpose for using IgG sensitized cells.	
39.06	Perform methods of adsorption and elution techniques.	
39.07	Perform procedures for identification of multiple antibodies in recipient specimen including enzyme enhancement, dosage, temperature, and complement binding.	
39.08	Determine appropriate dosage of Rh Immune Globulin based on test results.  	
39.09	Interpret the preliminary investigation results to determine whether a transfusion reaction has occurred.	
40.0	Demonstrate and understanding of advanced principles of molecular diagnostics.  The student will be able to:	
40.01	Describe the steps used to prepare genomic DNA from blood and buccal cells.	
40.02	Describe the process of Fluorescent In-Situ Hybridization (FISH).	
40.03	Compare and contrast real time PCR and conventional PCR.	

Additional Information

Laboratory Activities

Laboratory investigations that include scientific inquiry, research, measurement, problem solving, emerging technologies, tools and equipment, as well as, experimental, quality, and safety procedures are an integral part of this career and technical program/course. Laboratory investigations benefit all students by developing an understanding of the complexity and ambiguity of empirical work, as well as the skills required to manage, operate, calibrate and troubleshoot equipment/tools used to make observations. Students understand measurement error; and have the skills to aggregate, interpret, and present the resulting data. Equipment and supplies should be provided to enhance hands-on experiences for students.

Clinical learning experiences in a clinical laboratory and related areas are an integral part of this program.  Clinical learning experiences should reflect the full breadth of responsibilities expected of a Medical Laboratory Technician and should include appropriate experience in each of the areas of the laboratory described herein.  The specified length for each of the courses listed is inclusive of clinical experience for each of the respective laboratory sections.

Special Notes

This program meets the Department of Health HIV/AIDS Domestic Violence and Prevention of Medical Errors education requirements.  Upon completion of this program, the instructor will provide a certificate to the student verifying that these requirements have been met.

If students in this program are seeking a licensure, certificate, or registration through the Department of Health, please refer to 456.0635, F.S. for more information on disqualification for a license, certificate, or registration through the Department of Health. 

The Core should be taken first in the program.  Following the successful completion of the core, the student is eligible to take the National Health Care Foundation Skill Standards Assessment with instructor approval and the completion of a portfolio.

General Education Course Requirements for AS and AAS Degrees

State Board of Education Rule 6A-14.030(4), F.A.C., identifies 15 credit hours as the minimum amount of general education coursework required in the Associate of Science (AS) degree and the Associate of Applied Science (AAS) degree.  In addition, Rule 6A-14.0303, F.A.C., implements s. 1007.25, F.S., and requires students entering a technical education degree program in the 2022-2023 academic year, and thereafter, to complete at least one identified core course in each subject area as part of the general education course requirements (15 credit hours total) before a degree is awarded) The core subject areas include: 
•	Communication. 
•	Humanities.
•	Mathematics. 
•	Natural Sciences.
•	Social Sciences.

Career and Technical Student Organization (CTSO)

HOSA: Future Health Professionals is the co-curricular career and technical student organization providing leadership training and reinforcing specific career and technical skills. Career and Technical Student Organizations provide activities for students as an integral part of the instruction offered. 

Accommodations

Federal and state legislation requires the provision of accommodations for students with disabilities to meet individual needs and ensure equal access. Postsecondary students with disabilities must self-identify, present documentation, request accommodations if needed, and develop a plan with their counselor and/or instructors. Accommodations received in postsecondary education may differ from those received in secondary education. Accommodations change the way the student is instructed. Students with disabilities may need accommodations in such areas as instructional methods and materials, assignments and assessments, time demands and schedules, learning environment, assistive technology and special communication systems. Documentation of the accommodations requested and provided should be maintained in a confidential file.